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2 498 996

(13)

(51)

IPC

C07K14/435(2006-01-01)

C07K19/00(2006-01-01)

C12N15/12(2006-01-01)

C12N15/62(2006-01-01)

C12N15/63(2006-01-01)

C12N5/10(2006-01-01)

C12Q1/02(2006-01-01)

G01N21/64(2006-01-01)

(21) (22)

Application

2012126840/10, 2012-06-27

(24)

Start date

2012-06-27

(22)

Actual filing date

2012-06-27

(45)

Published

2013-11-20

(72)

Inventor

Belousov Vsevolod VadimovichMishina Natalija MikhajlovnaChudakov Dmitrij Mikhajlovich

(73)

Holder

Federal'Noe Gosudarstvennoe Bjudzhetnoe Uchrezhdenie Nauki Institut Bioorganicheskoj Khimii Im. Akademikov M.M. Shemjakina I Ju.A. Ovchinnikova Rossijskoj Akademii Nauk Ran)

CHIMERIC PROTEIN BEING FLUORESCENT BIOSENSOR FOR SIMULTANEOUS DETECTION OF HYDROGEN PEROXIDE AND PHOSPHATIDYL INOSITOL-3,4,5-TRIPHOSPHATE, NUCLEINIC ACID CODING SUCH PROTEIN, EXPRESSION CASSETTE AND EUCARIOTIC HOST CELL Russian patent published in 2013 - IPC C07K14/435 C07K19/00 C12N15/12 C12N15/62 C12N15/63 C12N5/10 C12Q1/02 G01N21/64

Abstract RU 2498996 C1

FIELD: biotechnologies.

SUBSTANCE: proposed chimeric protein with SEQ ID NO:02 is fluorescent biosensor, built on the basis of HyPer protein and mutant of PH-domain of Btk tyrosine kinase.

EFFECT: proposed inventions allow performing simultaneous monitoring of product of hydrogen peroxide and phosphatidyl inositol-3,4,5-triphosphate in a living cell.

4 cl, 4 dwg, 3 ex

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RU 2 498 996 C1

Authors

Belousov Vsevolod Vadimovich

Mishina Natalija Mikhajlovna

Chudakov Dmitrij Mikhajlovich

Dates

2013-11-20—Published

2012-06-27—Filed