FIELD: chemistry.
SUBSTANCE: claimed invention relates to field of biology and chemistry and deals with isolated nucleic acid, coding fluorescent protein with biosensor properties, expression cassettes, providing expression of said fluorescent protein, cells, producing said protein, and peculiarly fluorescent protein with biosensor properties. Obtained fluorescent protein has amino acid sequence, given in SEQ ID NO:4, and intended for changing NAD+/NADH ratio inside cells by increasing signal with displacement of NAD+/NADH ratio towards decrease of NADH concentration.
EFFECT: claimed invention makes it possible to carry out analysis of processes in cell in real time mode.
4 cl, 6 dwg, 4 ex
| Title |
Year |
Author |
Number |
| ISOLATED NUCLEIC ACID CODING FLUORESCENT BIOSENSOR FOR DETECTION OF HYDROGEN PEROXIDE, EXPRESSION CASSETTE, CELL THAT PRODUCES BIOSENSOR, ISOLATED FLUORESCENT BIOSENSOR FOR DETECTION OF HYDROGEN PEROXIDE, ISOLATED NUCLEIC ACID THAT CODES FLUORESCENT BIOSENSOR, EFFICIENTLY FUSED WITH NUCLEIC ACID THAT CODES SIGNAL OF INTRACELLULAR LOCALISATION |
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|
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|
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|
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|
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2013 |
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|
RU2535981C1 |
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2009 |
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|
RU2434943C2 |
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2011 |
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|
RU2458129C1 |
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2011 |
- Luk'Janov Konstantin Anatol'Evich
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|
RU2491342C1 |
| MODIFIED GENETICALLY CODED PHOTOSENSITIZER |
2013 |
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|
RU2599443C2 |
| NOVEL FLUORESCENT PROTEINS FROM ENTACMAEA QUADRICOLOR AND METHOD OF OBTAINING SAID PROTEINS |
2007 |
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|
RU2395581C2 |
