FIELD: chemistry; biotechnology.
SUBSTANCE: method of culturing the strain Rhodococcus rhodochrous M33 provides the use of a culture medium, which is based on 12-60 mM phosphate buffer in demineralised water, which meets the cell requirements in phosphor. The value of the pH is kept in the interval from 5.5 to 9.0. After all the glucose provided has been used then acetic acid and/or its water soluble salt as the new source of carbon. The medium for cultivating the strain includes 7.9 g/l Na2HPO4x12H2O, 1.8 g/l KH2PO4, 0.02-0.04 g/l CoCl2x6H2O, 1.0 g/l MgSO4x7H2O, 0-10 g/l corn steep-water concentrate, 20-90 g/l glucose, 4-20 g/l urea, demineralised water. The medium is used in the following way. Nitrile hydrate activity is 7169 units/millilitre.
EFFECT: increased removal of biomass with high nitrilehydrase activity.
19 cl, 3 tbl, 7 ex
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