BACTERIAL STRAIN RHODOCOCCUS AETHERIVORANS OF RUSSIAN CLASSIFICATION OF MICROORGANISMS BKM AC-2610D - PRODUCER OF NITRILE HYDRASE, METHOD OF ITS CULTIVATION AND METHOD OF PRODUCTION OF ACRYLAMIDE Russian patent published in 2014 - IPC C12N1/20 C12N9/78 C12P13/00 C12R1/01 

FIELD: biotechnology.

SUBSTANCE: bacterial strain Rhodococcus aetherivorans of Russian classification of microorganisms BKM Ac-2610D is proposed. The bacterial strain is different in growth on the common organic-mineral medium at high nitrile hydrase activity, reaching 332 U/mg at 20°C or 521 U/mg at 25°C. The nitrile hydrase of the bacterial strain Rhodococcus aetherivorans of Russian classification of microorganisms BKM Ac-2610D is thermostable. Also the method of culturing this strain is provided. The cells of the strain are inoculated on the slant meat-and-peptone agar and grown for 24-48 hours. Then the biomass is washed with sterile saline solution with pH 7.0-7.4. The resulting suspension is inoculated to the first container with the nutrient medium. The process is carried out for 24-48 hours at 28-30°C, with the circumferential stirring at a speed of 140-160 rev/min until obtaining the optical density of the suspension of 2-16 units at 540 nm and the magnitude of the optical layer of 5 mm. The resulting suspension is inoculated to the second container, which volume is 10-100 times greater than that of the first container. The value of optical density in the second container is adjusted to 0.1-0.3. The strain is cultured for 48-120 hours at 26-31°C, the aeration of 0.5-1.0 the air volume/medium volume per minute until the value of optical density reaches 36-40 and pH 7.5-7.8. The resulting biomass is separated. Also the method of production of acrylamide is provided. Hydration of acrylonitrile is carried out with the acrylonitrile concentration not exceeding 0.5%. The hydration is carried out using the biomass of bacterial strain Rhodococcus aetherivorans of Russian classification of microorganisms BKM Ac-2610D based on the order of 400-500 g of dry weight of strain per 1 ton of the final product - acrylamide with the concentration of 45-49%. The inventions enable to obtain cells of Rhodococcus aetherivorans of Russian classification of microorganisms BKM Ac-2610D of 10-18 g/l with the nitrile hydrase enzyme activity of 250-332 U/mg.

EFFECT: improvement of strain quality.

5 cl, 6 ex, 1 tbl