FIELD: chemistry; biotechnologies.
SUBSTANCE: method of producing immobilised biocatalyst includes trapping of Rhodococcus type microorganism cells of nitrile-hydrase activity on carbon carriers by delivering cellular suspension through the column, filled with the carrier, or by joint centrifugation of cells and carriers. Herewith carbon carriers are active carbons made of vegetative and polymer raw materials with porous structure parameters, including total pore space 0.4-1.8 cm3/g and micropore volume concentration 45-92%. Produced biocatalyst is used in method to produce aqueous amide solutions by carboxylic acid nitrile hydration.
EFFECT: high amide yield and increased time for amide synthesis biocatalyst application.
4 cl, 2 tbl, 5 ex
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