FIELD: chemistry, biochemistry.
SUBSTANCE: invention refers to biotechnology and represents method of solution analysis for homocysteine and/or cystathionine through enzymatic cyclic conversion. The analysis includes contacting stages of homocysteine and/or cystathionine containing solution with CBS, L-serine and CBL and reducing agent within time sufficient for homocysteine to cystathionine cyclic coversion catalysis deriving pyruvate and ammonia; reaction mixture is evaluated for pyruvate and/or ammonia. These findings are used to evaluate homocysteine and/or cystathionine content in solution. The first contacting stage takes at least 15 minutes. Besides invention covers analysis set.
EFFECT: reduced analysis time and higher sample throughput.
23 cl, 16 dwg, 34 tbl, 16 ex
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