ESCHERICHIA COLI BACTERIUM FOR MAKING 1,2-PROPANEDIOL, METHOD FOR MAKING THEREOF, METHOD FOR MAKING 1,2-PROPANEDIOL Russian patent published in 2010 - IPC C12N15/01 C12N9/02 C12N1/21 C12P7/18 C12P7/28 

FIELD: medicine.

SUBSTANCE: method for making Escherichia coli bacterium for producing 1,2-propanediol of an initial modified bacterium with the tpiA, gloA, aldA and aldB gene deletion, involves the cultivation of said initial modified bacterium in an appropriate culture medium containing a carbon source with applying the increasing potencies in such a manner that the culture medium preserves only the microorganisms with the growth rate being equal or exceeding the applied potency, for evolving said initial modified bacterium of one or more genes involved in a biosynthetic pathway of DHAP to methylglyoxal, then to 1,2-propanediol to produce evolving genes coding enzymes exhibiting higher activity of '1,2-propanediolsynthase', selection and recovery of one or more Escherichia coli bacteria exhibiting higher activity of '1,2-propanediolsynthase'. Also, the invention concerns initial microorganisms and thereby produced evolving microorganisms and the method for making 1,2-propanediol and acetone by cultivation of evolving microorganisms.

EFFECT: more efficient 1,2-propanediol production.

19 cl, 3 dwg, 3 tbl, 7 ex