FIELD: chemistry.
SUBSTANCE: method for the preparation of L-methionine in which O-phospho-L-homoserine (OPHS) and methanethiol are enzymatically converted to L-methionine and H3PO4 according to the reaction scheme: O-phospho-L-homoserine + CH3-SH <=> L-methionine + H3PO4. This enzymatic conversion is achieved by using OPHS-dependent methionine synthase, which is a protein obtained from cystathionine-gamma synthase (CF 2.5.1.48) as a result of a mutation. OPHS-dependent methionine synthase is a protein selected from the group consisting of (a) a protein comprising any of the amino acid sequences SEQ ID NO: 6 to 29; (b) a protein having a sequence identical to at least 90% of any one of SEQ ID NOs: 6 to 29, and having enzymatic activity of converting OPHS and methanethiol to L-methionine and H3PO4. Also disclosed is the use a protein having enzymatic activity of converting OPHS and methanethiol to L-methionine and H3PO4, for converting OPHS and methanethiol to L-methionine and H3PO4. Use of the method of producing L-methionine enables to produce S-adenosyl methionine, cysteine, glutathione, as well as 2-oxo-4-methylthiobutanoate.
EFFECT: group of inventions enables to efficiently convert OPHS + CH7-SH into L-methionine.
8 cl, 7 dwg, 3 tbl, 6 ex
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Authors
Dates
2018-04-17—Published
2013-10-25—Filed