METHOD FOR ENZYMATIC PRODUCTION OF L-METHIONINE FROM O-PHOSPHO-L-HOMOSERINE AND METHANETHIOL Russian patent published in 2018 - IPC C12P13/12 C12N9/10 C12N15/09 C12N15/54 C12N15/64 C12P21/02 

FIELD: chemistry.

SUBSTANCE: method for the preparation of L-methionine in which O-phospho-L-homoserine (OPHS) and methanethiol are enzymatically converted to L-methionine and H₃PO₄ according to the reaction scheme: O-phospho-L-homoserine + CH₃-SH <=> L-methionine + H₃PO_4. This enzymatic conversion is achieved by using OPHS-dependent methionine synthase, which is a protein obtained from cystathionine-gamma synthase (CF 2.5.1.48) as a result of a mutation. OPHS-dependent methionine synthase is a protein selected from the group consisting of (a) a protein comprising any of the amino acid sequences SEQ ID NO: 6 to 29; (b) a protein having a sequence identical to at least 90% of any one of SEQ ID NOs: 6 to 29, and having enzymatic activity of converting OPHS and methanethiol to L-methionine and H₃PO₄. Also disclosed is the use a protein having enzymatic activity of converting OPHS and methanethiol to L-methionine and H₃PO₄, for converting OPHS and methanethiol to L-methionine and H₃PO₄. Use of the method of producing L-methionine enables to produce S-adenosyl methionine, cysteine, glutathione, as well as 2-oxo-4-methylthiobutanoate.

EFFECT: group of inventions enables to efficiently convert OPHS + CH₇-SH into L-methionine.

8 cl, 7 dwg, 3 tbl, 6 ex