FIELD: chemistry; biochemistry.
SUBSTANCE: present invention pertains to biotechnology and molecular biology. Designed are specific oligonucleotide primers, with activity of direct and reverse primers in amplification reaction, with the following structure: 5'-GCAGGTTAATGAATAGCGTTGGTG-3'-CimMBP1s 5'-CAAACATCAGTCCGTAATCGTGTG-3'-CimMBP2as, for identifying, in a short period of time with high sensitivity and specificity, C.immitis and C.posadasii, using polymerase chain reaction method in biological material and environmental media. The invention can be used in medicine for identifying genetic material of stimulants of coccidioidomycosis Coccidioides immitis and Coccidioides posadasii in samples for diagnosis in practical health care or the federal service for heath and consumer rights, as well as in scientific research.
EFFECT: design of oligonucleotide primers, which can be used in medicine for identifying genetic material of stimulants of coccidioidomycosis Coccidioides immitis and Coccidioides posadasii in samples for diagnosis in practical health care or the federal service for heath and consumer rights, as well as in scientific research.
2 dwg, 3 ex
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