FIELD: chemistry.
SUBSTANCE: claimed is oligonucleotide probe with complementary terminal sequences of "molecular beacon" type, providing fluorescent detection in identification of coccidioidomycosis causative agent Coccidioides posadasii by PCR method with oligonucleotide primers CpSOW82s/CpSOWS2as, complementary to fragment of gene SOWgp82 C. posadasii. Probe has structure 5'(ROX)-CGCGGAGATGACTGTCGAGTGGCGCG-(BHQ2)3', where ROX -is fluorescent dye carboxy-X-rhodamine, BHQ2 is fluorescent extinguisher.
EFFECT: invention makes it possible to detect DNA in pure culture and biological material in short term with high sensitivity and specificity.
1 dwg, 1 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENT-LABELLED PROBE FOR IDENTIFYING DNA AGENTS OF COCCIDIOIDOMYCOSIS COCCIDIOIDES IMMITIS AND COCCIDIOIDES POSADASII | 2015 |
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