METHOD OF OBTAINING DIHYDROQUERCETIN Russian patent published in 2009 - IPC A61K36/00 B01D15/08 B01D15/42 

FIELD: chemistry.

SUBSTANCE: present invention pertains to chromatographic separation of bioflavonoids. Aliphatic alcohol is extracted from hogged larch wood. The alcohol is distilled and at least one cycle of chromatographic separation of the target product on a column filled with hydrophobic sorbent, is carried out, with elution of the sorbent by the alcohol-containing mobile phase. The extraction agent and mobile phase used is an aqueous solution of the same alcohol, chosen from a group comprising: methyl, ethyl and isopropyl. The chromatographic separation cycle is carried out in a temperature-controlled column at 30-50°C in two stages. At the first stage, concentration of alcohol in the solution, deposited on the column, and in the mobile phase is the same and equals 20-40% vol. At the second stage, the solution is deposited on the column with 1-20% vol. concentration of alcohol in the solution. Elution of the sorbent is carried out with alcohol concentration in the mobile phase at least, 3% higher than concentration of alcohol in the solution, from which depositing was carried out, but higher than 30% vol. The eluate obtained from chromatography is evaporated and the residue is dried. The invention allows for obtaining high output of product with high degree of purity.

EFFECT: obtaining high output of product with high degree of purity.

4 cl, 3 ex