FIELD: chemistry.
SUBSTANCE: present invention pertains to chromatographic separation of bioflavonoids. A solution of dihydroquercetin-containing raw material in an aqueous solution of aliphatic alcohol C1-C3 is obtained. At least one chromatography cycle is carried out with depositing a sample of the obtained solution on a column, filled with hydrophobic sorbent. The mobile phase is eluted. The sample is deposited at 1-20% vol. concentration of alcohol in the solution. The mobile phase used is an aqueous solution of the same alcohol with 3% more concentration than concentration of alcohol in the solution from which the sample is deposited, but not exceeding 30% vol. The eluate obtained from chromatography is evaporated and the residue is dried.
EFFECT: obtaining high output of product with high degree of purity.
3 cl, 3 ex
Title | Year | Author | Number |
---|---|---|---|
METHOD OF OBTAINING DIHYDROQUERCETIN | 2007 |
|
RU2349331C1 |
METHOD OF PRODUCING DIHYDROQUERCETIN | 2010 |
|
RU2435766C1 |
METHOD OF CHROMATOGRAPHIC PURIFICATION OF BUSERELIN | 2007 |
|
RU2332248C1 |
METHOD FOR CHROMATOGRAPHIC EXTRACTION AND PURIFICATION OF IMMUNOGLOBULINS | 2018 |
|
RU2694620C1 |
METHOD OF PURIFYING GOSERELIN | 2015 |
|
RU2578414C1 |
CHROMATOGRAPHIC METHOD OF EREMOMYCIN PURIFICATION | 2006 |
|
RU2333963C1 |
METHOD OF CHROMATOGRAPHY EXTRACTION AND CONCENTRATION OF ANTHOCYANS | 2020 |
|
RU2727130C1 |
METHOD FOR CHROMATOGRAPHIC EXTRACTION OF IMMUNOGLOBULIN | 2010 |
|
RU2467783C2 |
METHOD FOR PURIFICATION OF RECOMBINANT IL-36RA (EMBODIMENTS) | 2018 |
|
RU2700751C1 |
METHOD OF PURIFYING TRIPTORELIN | 2015 |
|
RU2585105C1 |
Authors
Dates
2009-03-20—Published
2007-10-15—Filed