METHOD OF DETERMINATION OF FOODSTUFF CONTAMINATION WITH PATHOGENIC BIOLOGICAL AGENTS IN EXTREME SITUATIONS Russian patent published in 2009 - IPC C12Q1/00 C12Q1/68 G01N33/569 

FIELD: medicine; microbiology.

SUBSTANCE: invention concerns area of medical microbiology and can be used at carrying out of the laboratory control of the foodstuffs on contamination pathogenic biological agents in the conditions of extreme situations. In the invention the new technological scheme of carrying out of the laboratory control of the foodstuffs where preparation of assays spend to two stages is offered: the first stage - sample preparation - depending on density, fat content and fluidity of a product it is exposed, accordingly: to weighing, allocating 25 g (ml) of a product with the subsequent crushing, to transfer in a liquid phase in volume of 50-100 ml by means of normal saline solution addition, and at water research in number of 500 ml filter last through membranous filters; the second stage provides division of assay into parts: on 2 ml of assays separate for researches by methods of fluorescent antibodies (MFA), in the reaction of an indirect hemagglutination (RIHA), in reaction of a sintering of volume (RSV), enzyme immunoassay (EIA), polimerase chain reaction (PCR) and a bacteriological method and on 5 ml of assays take for research by a biological method, infecting biotrial animals. Research of assays by the express or accelerated methods (MFA, RIHA, RSV, EIA, PCR) spend within 4-10 hours, and research by a biological method - within 48-120 hours with use of selective nutrient mediums, such as the ADET-agar diagnostic elective tularemic, ADEA-agar diagnostic elective anthracic, EEDC-environment elective diagnostic choleraic, PYM-37 plague yeast medium.

EFFECT: unification of carrying out of the laboratory analysis of the foodstuff; parallel research of 25 assays, which preparation takes 1,5-2 hours.

6 cl, 10 tbl