FIELD: biotechnologies; medicine.
SUBSTANCE: claimed is method of diagnostics of hereditary predisposition to throbophilia, which includes isolation of DNA, amplification of sections of MTHFR genes in humans, V factor of blood clotting (FV) and prothrombin (FII) by carrying out polymerase chain reaction using up to three pairs of specially selected oligonucleotide primers, processing amplification fragments with restriction TaqI endonuclease and determination of obtained fragments size by electrophoresis. In discovering polymorphic replacements C677→T in MTHFR gene, G1691→A in gene FII and G20210→A in gene FII, hereditary predisposition to thrombophilia is diagnosed.
EFFECT: invention can be used in medicine as easier and more accurate method of diagnosing individual predisposition to thrombophilia.
1 dwg, 4 tbl, 2 ex
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