FIELD: medicine.
SUBSTANCE: histochemical aluminium detection is ensured with aurintricarboxylic acid solution at temperature 75°C during 5-10 min with using buffer mixture of pH 5.2, containing ammonium chloride, ammonium acetate and HCl, thereafter differentiated in ammonium carbonate solution during 3-5 s and additionally coloured with 1% methylene blue in water. Prior to colouring, the histochemical sections are prepared, with 3% ascorbic acid solution, then exposed in buffer mixture and coloured with aurintricarboxylic acid on water bath.
EFFECT: higher selectivity of method of aluminium detection found with iron ions both in organs and tissues of animals.
Title | Year | Author | Number |
---|---|---|---|
METHOD OF DETERMINATION OF PRESENCE OF ALUMINUM IN THE BIOLOGICAL OBJECTS | 2003 |
|
RU2265842C2 |
METHOD OF DETECTING MICROBES IN TISSUE | 0 |
|
SU1411622A1 |
METHOD OF DETECTING MAST CELLS ON HISTOLOGICAL PREPARATIONS OF THE HUMAN HEART | 2022 |
|
RU2798117C1 |
METHOD OF HYSTOCHEMICAL DETECTION OF METHACIDE IN LIVER IN CASE OF POISONING WITH POLYMER-BASED SURROGATE ALCOHOL | 2010 |
|
RU2433409C1 |
METHOD OF REPRODUCTIVE RESPIRATORY SYNDROME DIAGNOSTICS IN PIGS USING INDIRECT IMMUNOHISTOCHEMICAL ANALYSIS ON THE BASIS OF MONOCLONAL ANTIBODIES | 2016 |
|
RU2645114C2 |
METHOD FOR DETECTING NERVE FIBERS OF ADRENERGIC AND CHOLINERGIC NATURE IN TISSUE | 2004 |
|
RU2256179C1 |
METHOD FOR ACCELERATED STAINING OF HISTOLOGICAL SPECIMEN FOR IDENTIFYING TUBERCULOSIS MYCOBACTERIA | 2013 |
|
RU2530574C2 |
METHOD FOR DETECTING ALKALINE PHOSPHATASE IN IMMUNOCOMPETENT ORGANS IN ANIMALS | 2000 |
|
RU2198404C2 |
HISTOCHEMICAL METHOD FOR DETECTING PROTEINS BASED ON COOMASSIE AND METHOD FOR PREPARING AND STORING HISTOLOGICAL PREPARATIONS | 2021 |
|
RU2790868C1 |
METHOD OF CARTILAGE AND OSSEOUS TISSUE STAINING | 1991 |
|
RU2033610C1 |
Authors
Dates
2009-04-27—Published
2007-04-25—Filed