CERULOPLASMIN RECOVERY METHOD Russian patent published in 2009 - IPC A61K35/16 A61K38/48 

FIELD: medicine.

SUBSTANCE: invention refers to medicine, namely to ceruloplasmin recovery, and can be used in making enzyme blood-plasma medicines. The disclosed method includes dissolution of feed stock in acetate buffer, enzyme sorption in an ion-exchange reactor with functional DEAE group, elution and following purification. Said ion-exchange reactor with functional DEAE group represents a sorbent on fixed styroldivinylbenzene matrix with co-graft DEAE group. And in purification, at first the dissolved enzyme is treated with a solvent-detergent mixture. Then the treated enzyme is immobilised on sulphopropylcation sorbent, washed in acetate buffer containing sodium chloride. The purified enzyme is eluted, while the feed stock is dissolved in acetate solution. The enzyme is sorbed in the ion-exchange reactor wherein as such sorbent on fixed styroldivinylbenzene matrix with co-graft DEAE group is applied. The enzyme is eluted and purified. In purification, the enzyme solution is treated with a solvent-detergent mixture, immobilised on sulphopropylcation sorbent, washed in acetate buffer containing sodium chloride. The purified enzyme is eluted.

EFFECT: method ensures virus safety of recovered medical product ceruloplasmin and allows improving end product yield.

9 cl, 2 ex