FIELD: biotechnology.
SUBSTANCE: invention relates to methods of obtaining, isolating and purifying recombinant proteins, in particular to obtaining a highly purified preparation of S. marcescens correctly folded recombinant endonuclease.
EFFECT: method for obtaining Serratia marcescens recombinant endonuclease produced in E. coli including the steps of washing and dissolving inclusion bodies, refolding and protein chromatography, characterized by the following: the refolding is carried out by dilution at a concentration of refolded protein from approximately 0.3 to 0.5 g/l.
9 cl, 2 ex, 7 dwg, 4 tbl
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