PROTEIN RECOVERY AND PURIFICATION Russian patent published in 2011 - IPC C12N15/82 C12N15/62 C07K1/14 

FIELD: medicine.

SUBSTANCE: presented recombinant proteins contain a sequence inducing protein bodies and fused with a sequence of a concerned product. The method implies producing an aqueous homogenate of transformed host cells which express fused protein in the form of recombinant protein body like aggregates (RPBLA) showing the density approximately 1.1 to 1.35g/ml. The homogenate is centrifuged with producing fractions of various densities, or in the absence of an additional solution providing volume, or with using a layer of a certain density which is less than the density of RPBLA. As a result, the fractions containing relatively lowered concentration of RPBLA and a fraction containing relatively higher concentration of RPBLA are produced. The fraction of the lowered concentration of RPBLA is separated from the fractions of relatively higher concentration of RPBLA. Then said fused protein is purified.

EFFECT: easy-to-implement, immediate and effective purification of recombinant proteins.

15 cl, 8 dwg, 1 tbl, 18 ex