FIELD: biotechnologies.
SUBSTANCE: differentiation of four Trichobilharzia species: T. szidati, T.regenti, T.franki and T.sp.var.narochanica is performed by amplification of sections of sequence of nuclear ribosomal DNA (rDNA) in specimens of reproductive helmints, their larval stage and/or on rDNA of fresh-water mollusks of Lymnaeidae family, which are infected with the above helmints by PCR and four oligonucleotide primers of the following composition: F: 5'-CTTTCCATCTATCACGATGCACT-3' R1: 5'-ATGATAATGTGCATAACACACC-3' R2: 5'-GCCGTTTATTTATATGTATGTG-3' R3: 5'-CAAGCCGTTTATTWATATATAACGG-3'. The obtained amplification products are visualised and differentiated and identified as per size (length); with that, one amplification fragment with the size of 255 base pairs is relevant for T.regenti, species, one fragment with the length of 316 base pairs is relevant for T.szidati species, two fragments with the size of 255 and 316 base pairs are relevant for T.sp.var.narochanica species, and amplification fragment with the size of 258 base pairs is detected only for T.franki species.
EFFECT: method allows simultaneous detection and species identification of fours types of ornithic schistosomes of Trichobilharzia species at different life stages.
2 dwg, 2 tbl, 2 ex
