METHOD OF COLLECTING FERTILISED EGGS (in vitro) FROM EXCITER Fasciola hepatica INTRA VITAM Russian patent published in 2015 - IPC C12Q1/02 G01N1/00 

Abstract RU 2556132 C1

FIELD: biotechnology.

SUBSTANCE: method comprises the steps: collecting from the bile ducts of liver of domestic and/or wild animals infected with fasciolas of only live adult F. hepatica. Placement them into individual tubes with filtered and centrifuged bile diluted with isotonic solution of sodium chloride 1:1. Exposure of tubes at t = 38-39°C if F. hepatica is from cattle, and t = 39-40°C if F. hepatica is from sheep and/or goats, under conditions of thermostat for 5 hours. Subsequent washing eggs in isotonic solution of sodium chloride.

EFFECT: invention enables to obtain up to 100 percent of fertilised eggs of Fasciola of species Fasciola hepatica and can be used for study in the laboratory or field experiments in solving fundamental and applied scientific tasks in the field of epizootiology, treatment and prevention of fascioliasis of domestic or wild animals.

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RU 2 556 132 C1

Authors

Pasechnik Valerij Evgen'Evich

Dates

2015-07-10Published

2014-08-08Filed