FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology and can be used in the food industry, as well as in production of detergent materials. A gene of new phospholipase (FvPLA2) is cloned from Fusarium venenatum, which belongs to a group of fungal and bacterial phospholipases XIII PLA2. Analysis has been of physicochemical properties and catalytic activity of the new phospholipase, in accordance with the results of which proposal is made for using FvPLA2 in baking bread, production of vegetable oil, making cheese, as well as in detergent compositions.
EFFECT: nucleotide sequence is defined, which codes FvPLA2 and its active form, through expression of which a recombinant product with phospholipase activity is obtained in a heterologous system.
8 cl, 6 tbl, 11 ex
| Title | Year | Author | Number |
|---|---|---|---|
| POLYPEPTIDES HAVING ENDOPEPTIDASE ACTIVITY AND POLYNUCLEOTIDES CODING SAME | 2011 |
|
RU2583293C2 |
| RECOMBINANT DEFENSIN EXPRESSION IN FILAMENTOUS FUNGI | 2006 |
|
RU2404990C2 |
| METHODS FOR ENHANCING THE DEGRADATION OR CONVERSION OF CELLULOSIC MATERIAL | 2006 |
|
RU2441912C2 |
| POLYPEPTIDES WITH ANTIMICROBIAL ACTIVITY AND POLYNUCLEOTIDES CODING THEM | 2005 |
|
RU2393224C2 |
| ANTIMICROBIAL POLYPEPTIDES FROM PSEUDOPLECTANIA NIGRELLA | 2002 |
|
RU2336278C2 |
| POLYPEPTIDES HAVING ANTIMICROBIAL ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME | 2006 |
|
RU2512525C2 |
| POLYPEPTIDES HAVING PROTEASE ACTIVITY AND NUCLEAR ACIDS ENCODING THE SAME | 2002 |
|
RU2318018C2 |
| POLYPEPTIDES WITH LYSOZYME ACTIVITY AND POLYNUCLEOTIDES CODING THEM | 2012 |
|
RU2619051C2 |
| POLYPEPTIDES EXHIBITING ANTIMICROBIAL ACTIVITY AND CODING POLYNUCLEOTIDES | 2006 |
|
RU2415150C2 |
| LIPOLYTIC ENZYME FOR USE IN BAKING | 2018 |
|
RU2763469C2 |
