FIELD: biotechnology.
SUBSTANCE: an isolated polypeptide having a lysozyme activity is presented. Isolated polynucleotide coding the said polypeptide is presented. Compositions for extraction of bacterial genomic DNA, composition of animal feed and animal feed supplement, containing the said polypeptide, are presented. This is the method for extracting DNA out of bacteria using the said polypeptide. An expression vector of a nucleic acid containing the said polynucleotide operably linked to regulatory sequences is presented. A recombinant bacteria or fungus host cell is introduced for producing the said polypeptide. It comprises of the said polynucleotide operably linked to regulatory sequences. A method for producing a polypeptide having lysozyme activity using the said host cells is presented.
EFFECT: group of inventions allows to obtain a lysozymic enzyme with an improved thermal stability, using which makes it possible to avoid a loss of enzyme activity at production stages combined with temperature exposure.
20 cl, 6 dwg, 15 tbl, 13 ex
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Authors
Dates
2017-05-11—Published
2012-11-23—Filed