FIELD: medicine; veterinary science.
SUBSTANCE: method involves fraction formalinisation and antigen sensitisation of sheep's erythrocytes at 70°C within 30 minutes. Said antigen is that prepared from cultivation of culture Anaplasma sp. Omsk on cells Vero and a nutrient growth-support medium Needle MEM with embryo serum, amino acids and antibiotics added, and recovery after eight-day incubation by alternating freezing and thawing, heating on a water bath at 70°C within 30 minutes and triple washing without 0.4% normal rabbit serum added at the last stage of preparation.
EFFECT: high specificity and activity in indirect hemagglutination test in cattle anaplasmosis.
3 tbl
| Title | Year | Author | Number |
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| METHOD OF OBTAINING ERYTHROCYTE DIAGNOSTICUM FOR REACTION OF INDIRECT HEMAGGLUTINATION IN CATTLE COCCIELLOSIS | 2009 |
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| METHOD FOR PRODUCTION OF ERYTHROCYTIC DIAGNOSTICUM FOR INDIRECT HEMAGGLUTINATION REACTION IN CATTLE MYCOPLASMOSIS | 2007 |
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| METHOD FOR MAKING ERYTHROCYTIC DIAGNOSTICUM FOR INDIRECT HEMAGGLUTINATION REACTION (IHR) IN PIG'S MYCOPLASMOSIS | 2007 |
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| METHOD FOR PREPARING ANTIBODY ERYTHROCYTE DIAGNOSTICUM FOR INDIRECT HEMAGGLUTINATION TEST (IHT) FOR PURPOSES OF BRUCELLA INDICATION IN PATHOLOGIC MATERIAL | 2012 |
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| METHOD OF PRODUCTION OF R-BRUCELLAR ERYTHROCYTIC ANTIGEN FOR INDIRECT HEMAGGLUTINATION TEST (IHT) | 2011 |
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| METHOD OF PRODUCING A BRUCELLOUS ERYTHROCYTE ANTIGEN FOR THE INDIRECT HEMAGGLUTINATION REACTION (IHAR) | 2019 |
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| METHOD FOR PRODUCING ERYTHROCYTIC ANTIGEN FOR SERUM DIAGNOSTICS OF BRUCELLOSIS | 2008 |
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| METHOD FOR PRODUCING BRUCELLAR ERYTHROCYTIC DIAGNOSTICUM FOR INDIRECT HEMAGGLUTINATION TEST (IHGT) | 2016 |
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| METHOD FOR OBTAINING ANTITULAREMIC SERUM AND METHOD FOR OBTAINING A DRY ERYTHROCYTIC TULAREMIC IMMUNOGLOBULIN TEST KIT | 2002 |
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