METHOD FOR MAKING ERYTHROCYTIC DIAGNOSTICUM FOR INDIRECT HEMAGGLUTINATION REACTION (IHR) IN PIG'S MYCOPLASMOSIS Russian patent published in 2009 - IPC G01N33/569 

Abstract RU 2361218 C1

FIELD: medicine.

SUBSTANCE: invention refers to mycoplasmoses laboratory diagnostic techniques and can be used in veterinary medicine. Method for making erythrocytic disgnosticum for indirect hemagglutination reaction (IHR) in pig's mycoplasmosis consists from fractional formalinisation of sheep's erythrocytes and sensitisation with mycoplasmosis antigen at 70°C within 30 minutes. And for sensitisation, erythrocytes are used being loaded with sensitine made of mixed mycoplasma cultures (M.hyosynoviae, M.hyorhinis and Ureaplasma sp.) taken in equal proportions and heated on water bath at 70°C within 30 minutes. Thereafter the diagnosticum is triply washed with a phosphate-buffer salt solution with PH-7.2.

EFFECT: higher specificity and activity of erythrocytic disgnosticum in indirect hemagglutination reaction (IHR) in pig's mycoplasmosis.

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RU 2 361 218 C1

Authors

Krasikov Aleksandr Panteleevich

Zhongolovich Anna Evgen'Evna

Dates

2009-07-10Published

2007-09-21Filed