FIELD: chemistry.
SUBSTANCE: present invention refers to analytical chemistry and can be used for quantative determination of methionine in water solutions by spectrophotmetric method. The method includes the preparation of standard methionine solutions, determination of optical density at characteristic wavelength, plotting of the calibration function for standard methionine solutions (optical density against concentrations at characteristic wavelength), determination in the analysed methionine solution of the optical density and methionine concentration according to optical density at characteristic wavelength using the calibration function. The determination of the characteristic wavelength is implemented in ultraviolet spectral region according to the differential absorption in coordinates λ=f(ΔA) whereat ΔA=Afollowing-Aprevious.
EFFECT: enhancing of determination reliability, accuracy and selectivity as well as simplifying and time decrease of the determination.
3 dwg
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Authors
Dates
2009-09-10—Published
2008-05-13—Filed