FIELD: medicine.
SUBSTANCE: invention refers to laboratory diagnostics and can be used for evaluation of phagocytic activity of neutrophils in human peripheral blood. Phagocytosis objects are recombinant luminescent bacteria, namely microorganisms Escherichia coli with cloned luxCDABE genes Photobacterium leiognathii, pre-opsonised during 10 min with normal human immunoglobulin in end concentration 6-10 mg/ml. Luminole is additionally introduced to the sample to end concentration 10-3 M. Intensity of luminole-dependent phagocytic chemoluminescence and bioluminescence of bacterial targets are counted separately by evaluating luminescence in two various spectral bands: <430 nm for evaluation of chemoluminescence and >540 nm for evaluation of bioluminescence.
EFFECT: possibility for complex evaluation of activation level of oxygen-dependent bactericidal phagocytic systems, and improved test accuracy.
1 ex, 1 tbl
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