FIELD: medicine.
SUBSTANCE: group of inventions refer to medical diagnostics and covers diagnostics of early myocardial infarction by direct determination of myoglobin from human myocardium. Said biosensor represents a disposable graphite electrode modified by colloidal gold and monoclonal antibodies to myoglobin from human myocardium. It is produced the way as follows. A three-contact graphite main electrode is covered with colloidal gold solution stabilised with didodecyl methylammonium bromide in chloroform. It is dried at room temperature. The electrode structure is modified by antibodies to myoglobin from human myocardium by covering the main graphite electrode to myoglobin from human myocardium modified with didodecyl methylammonium bromide and colloidal gold. Then it is dried, settled at +2°÷+6°C, rinsed with water, processed with a blocking buffer, dried, settled at room temperature and rinsed with water.
EFFECT: possibility to determine myoglobin in aqueous solutions.
3 cl, 2 dwg, 5 ex
