ELECTROCHEMICAL BIOSENSOR FOR DIRECT RECORDING OF MYOGLOBIN BASED ON CARBON NANOTUBES AND MOLECULAR IMPRINTED POLYMER BASED ON O-PHENYLENEDIAMINE AND PREPARATION METHOD THEREOF Russian patent published in 2016 - IPC G01N33/543 

FIELD: chemistry.

SUBSTANCE: group of inventions relates to analytical chemistry, electrochemistry and medical diagnosis and can be used for diagnosing early stages of myocardial infarction. Electrochemical biosensor for direct recording myoglobin in aqueous buffer solutions by measurement of peak height of electroreduction myoglobin by square-wave voltammetry is a graphite electrode, modified with carbon nanotubes and analogue of anti-myoglobin antibodies, which is an o-phenylenediamine polymer which is molecular-imprinted for myoglobin. Group of inventions also relates to a method of producing said biosensor, including modification of surface of graphite electrode with a suspension of carbon nanotubes, subsequent modification of obtained electrode by electropolymerisation of o-phenylenediamine in presence of myoglobin, performed on surface of nanotube-modified electrode, and incubation of obtained electrode in a solution containing ethanol and 0.25 M NaOH at ratio 2:1. Suspension of carbon nanotubes is produced by ultrasonic disintegration of carbon nanotubes in an organic solvent.

EFFECT: group of inventions provides high sensitivity of biosensor.

2 cl, 2 dwg, 2 ex