FIELD: medicine.
SUBSTANCE: there is offered method of electrochemical detection of specific biomolecules in a biological sample, and also a device for implementation thereof. In a measuring cell when stirred an immune complex is formed on an electrode surface; an electrode is washed with a buffer solution; marked antibodies are added. There is also provided contact of a biosensor with a reference biological sample; te cell is washed once more, current is detected on an exploring electrode with controlling temperature of the biological sample. The device for electrochemical detection of specific biomolecules comprises a measurement chamber accommodating an exploring electrode, an auxiliary electrode and a reference electrode, a temperature probe. Alternatively, the device has one more temperature probe mounted in close proximity to the exploring electrode.
EFFECT: method allows improving reliability of diagnostic results, extending range of detected objects.
6 cl, 3 ex, 3 dwg
Title | Year | Author | Number |
---|---|---|---|
METHOD OF QUANTITATIVE ELECTROCHEMICAL ANALYSIS OF BIOLOGICAL MOLECULES | 1998 |
|
RU2161653C2 |
METHOD OF BLOOD PLASMA PROXIMATE ANALYSIS FOR CARDIOMYOGLOBIN BY MEANS OF ELECTROCHEMICAL IMMUNOSENSOR | 2009 |
|
RU2425382C1 |
DEVICE FOR DETERMINATION OF BIOLOGICALLY ACTIVE COMPOUNDS IN BIOLOGICAL FLUIDS AND PROCESS OF MANUFACTURE OF SENSITIVE ELEMENT | 1991 |
|
RU2032908C1 |
ELECTROCHEMICAL BIOSENSOR FOR DIRECT DETERMINATION OF MYOGLOBIN, METHOD FOR MAKING THEREOF | 2008 |
|
RU2367958C1 |
ELECTROCHEMICAL BIOSENSOR FOR DIRECT RECORDING OF MYOGLOBIN BASED ON CARBON NANOTUBES AND MOLECULAR IMPRINTED POLYMER BASED ON O-PHENYLENEDIAMINE AND PREPARATION METHOD THEREOF | 2015 |
|
RU2604688C2 |
METHOD OF EXPRESS DETERMINATION OF CARDIOMYOGLOBIN IN BLOOD PLASMA USING ELECTROCHEMICAL SENSOR BASED ON CARBON NANOTUBES AND MOLECULAR IMPRINTED POLY-O-PHENYLENEDIAMINE AS BIOAFFINITY REAGENT | 2016 |
|
RU2633086C1 |
ELECTROCHEMICAL METHOD FOR DETERMINING SPECIFIC ANTIBODIES IN BLOOD SERUM BY MEANS OF PROTEINS LABELED WITH METALS | 2002 |
|
RU2249217C2 |
METHOD FOR DETERMINATION OF GRAM-NEGATIVE PATHOGENIC BACTERIA IN ANALYSED MEDIUM | 2013 |
|
RU2542487C2 |
METHOD OF DETECTING PATHOGENIC MICROORGANISMS | 2009 |
|
RU2397243C1 |
METHOD OF DETERMINING CONTENT OF BACTERIA USING MODIFIED MAGNETIC NANOPARTICLES AS TAGS | 2015 |
|
RU2612143C2 |
Authors
Dates
2010-04-10—Published
2008-05-12—Filed