FIELD: chemistry.
SUBSTANCE: invention relates to clinical biochemistry and pertains to a method of extracting extracellular DNA from blood. Nonidet P-40 or triton X-100 is added to the analysed blood sample until final concentration of 0.5 to 1.5%. The mixture is incubated in ice for 1 to 15 minutes. Lysed blood is divided into supernatant and a nuclear fraction of cells by centrifuging. The supernatant is then treated with a RNA-hydrolysing enzyme and extracellular DNA is extracted from the obtained fraction using a fibre-glass sorbent.
EFFECT: use of the method simplifies the procedure of extracting extracellular DNA, simultaneously extract extracellular DNA, which is free and which is bonded to the surface of blood cells, from blood lysate, which allows for significantly increasing reliability and sensitivity of further amplification analysis for early detection of oncological and other diseases.
2 cl, 4 tbl, 4 ex
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|
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| DIAGNOSTIC AND MONITORING TECHNIQUE FOR ONCOLOGY DISEASES WITH USE OF BLOOD EXOSOMES | 2014 |
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