METHOD OF OBTAINING OVERALL FRACTION OF EXTRACELLULAR NUCLEIC ACIDS FROM BLOOD Russian patent published in 2015 - IPC G01N33/48 

FIELD: chemistry.

SUBSTANCE: method includes collecting blood in an anticoagulant solution, adding an equal volume of an elution buffer containing 1 M NaCl, 0.2 M NaHCO₃ (pH 9.3), 20 mM EDTA or a buffer containing 1 M NaCl, 20 mM EDTA. The obtained mixture is incubated at room temperature for 4-6 minutes while stirring, followed by separating the collected blood into plasma and a blood cell fraction by centrifuging and collecting the supernatant fluid, from which the overall fraction of extracellular nucleic acids (exNA) is extracted.

EFFECT: use of the invention shortens the duration of obtaining an overall fraction of exNA, increases the output of blood exNA, increases the sensitivity of detecting market NA, which are specific for cancerous diseases and pregnancy pathologies.

3 cl, 3 tbl, 4 ex