FIELD: medicine, namely molecular biology and oncology.
SUBSTANCE: invention relates to the field of medicine, in particular to molecular biology and oncology. A minimally invasive method for the diagnosis of high-grade serous ovarian adenocarcinoma based on the copy index of the SULT1E1, CYP1B1, and ESR1 genes is proposed. Extracellular DNA is isolated from blood plasma. The copy number of the SULT1E1, CYP1B1, and ESR1 genes relative to the GAPDH reference gene is determined by PCR-RV. The relative copy number of the gene (rC) is calculated using the formula rC=2-ΔCt. The obtained rC values are compared with the predictive copy interval. With values of rCCYP1B1 from 6.67*10-3 to 7.13*10-3, rCESR1 from 10.0*10-3 to 10.2*10-3 and rCSULT1E1 from 5.12*10-3 to 5.28*10-3, high-grade serous ovarian adenocarcinoma is diagnosed. With values of rCCYP1B1 from 3.19*10-3 to 3.81*10-3, rCESR1 from 1.91*10-3 to 2.09*10-3 and rCSULT1E1 from 2.23*10-3 to 2.57*10-3, the absence of malignant ovarian tumors is diagnosed.
EFFECT: invention provides the creation of a new, simple in execution, inexpensive and accurate method with unique highly specific primer sequences for the minimally invasive diagnosis of high-grade serous ovarian adenocarcinoma.
4 cl, 1 tbl, 2 ex
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