METHOD FOR INCREASE OF MELIOIDOSIS ANTIGENS PROTECTIVITY BY CYTOKINES Russian patent published in 2009 - IPC A61K39/07 A61K38/19 A61K38/21 A61K38/20 A61P31/04 

FIELD: medicine.

SUBSTANCE: invention is related to the field of medicine, in particular to microbiology and immunology and may be used in development of vaccine against melioidosis. Substance of method consists in the fact that animals are immunised with surface melioidosis complex, which consists of antigen 6 (AG6) and antigen d, which is injected to animals subcutaneously, twice, with interval of 10 days, in doses of 30 mcg by protein for mice or 150 mcg for rats. Simultaneously with primary immunisation recombinant interferon-γ - ingaron is injected in doses 8 ME for mice or 120 ME for rats, and in case of secondary immunisation, recombinant interleukin-2 - roncoleukin in doses of 0.6 mcg for mice or 10 mcg for rats, besides cytokines are injected to animals subcutaneously, daily, simultaneously with immunisation and in the next 2 days. In 21 days after primary immunisation animals are infected with 4-32 LD₅₀ of highly virulent strain 100 of melioidosis causative agent, in 30 days after infection parametres of animal lethality are identified.

EFFECT: application of invention makes it possible to increase protectivity of melioidosis antigens due to cytokine stimulation of primary and secondary immune response to these antigens.

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