METHOD FOR ISOLATION OF BIOACTIVE FRACTION (BAF) CONTAINING S-LIPOPOLYSACCHARIDES (S-LPS) FROM GRAM-NEGATIVE BACTERIA, BAF FOR PREVENTION AND TREATMENT OF DISEASES INDUCED BY ENDOTOXIC S-LPS PRODUCING GRAM-NEGATIVE BACTERIA, PHARMACEUTICAL COMPOSITION, METHODS FOR INDUCING OF PROTECTIVE IMMUNITY AND IMPROVING STATE IN PATIENT IN NEED OF IMMUNE STATUS INCREASING Russian patent published in 2005 - IPC

Abstract RU 2260053 C2

FIELD: biotechnology, medicine.

SUBSTANCE: invention relates to method for isolation of bioactive fraction (BAF) containing essentially S-lipopolysaccharides (S-LPS) from endotoxic S-LPS producing gram-negative bacteria, useful in prophylaxis and therapy. From endotoxic S-LPS producing gram-negative bacteria BAF is isolated that has in lipid A S-LPS molar ratio of D-glucoseamine and β-hydroxy acids selected from β-hydroxydecanic, β-hydroxydodecanic, β-hydroxytetradecanic and β-hydroxyhexadecanic acids of about 2:1-4, and molar ration of D-glucoseamine and high fatty acids connected via either amide or ester bond in lipid A S-LPS of about 2:3-7. Isolated BAF ins used in pharmaceutical composition to increase of patient immune status.

EFFECT: method for isolation from gram-negative bacteria bioactive fraction of S-LPS with high immunogenic properties, being capable to induce cytokine production with low pyrogenic and endotoxic properties.

41 cl, 4 dwg, 14 tbl, 24 ex

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RU 2 260 053 C2

Authors

Aparin P.G.

L'Vov V.L.

Elkina S.I.

Golovina M.Eh.

Shmigol' V.I.

Dates

2005-09-10—Published

2002-06-26—Filed