METHOD OF INDIRECT ESTIMATION OF STRAINS VIRULENCE OF PATHOGENIC BURKHOLDERIA ON GROUNDS OF CYTOPATHOGENICITY Russian patent published in 2013 - IPC C12Q1/00 

FIELD: biotechnology.

SUBSTANCE: as an object for indirect estimation apparently healthy leaves of the plant Peireskia aculeate are used, on which sterile surface the notches are made, the infecting doses of the strains Burkholderia pseudomallei and Burkholderia mallei under study in different concentrations are applied, placed on a wet swab and incubated at 32°C for 24-48 h. The extent of their cytopathogenicity is evaluated visually according to the damage to the leaf blade - maceration, ulceration and blackening. Simultaneously the level of virulence of strains of pathogens for laboratory animals is determined, and the extent of cytopathogenicity for P. aculeata is correlated with virulence for laboratory animals.

EFFECT: invention provides high sensitivity, reproducibility, versatility, ease and accessibility of the study.

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