FIELD: microbiology.
SUBSTANCE: glia cells are extracted, seeded, and cell is cultivated to produce monomolecular layer. Then cells are extracted and washed. Total RNA is extracted from them, reverse transcription is carried out, as well as amplification of produced DNA. Genes expression is assessed under action of tested compound.
EFFECT: invention makes it possible to accelerate screening of compounds, to search for new compounds with highest activity, which are potential neuroprotectors.
4 cl, 4 dwg, 1 tbl, 4 ex
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| METHOD OF SCREENING PHARMACOLOGICAL COMPOUNDS ACCORDING TO NEUROPROTECTIVE ACTIVITY | 2009 |
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| METHOD FOR SIMULATING TISSUE STRUCTURE OF HUMAN RETINA | 2012 |
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RU2690846C1 |
| PHARMACEUTICAL COMPOSITION FOR PREVENTION AND TREATMENT OF NEUROLOGIC DISEASES | 2007 |
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RU2335296C1 |
| METHOD OF PREPARING HUMAN EMBRYONAL NEURONS PREPARATION FOR CYTOTRANSFUSION | 1998 |
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| METHOD FOR ASSESSING NEUROPROTECTIVE PROPERTIES OF VITRO SUBSTANCES AND THE TEST SYSTEM FOR ITS IMPLEMENTATION | 2016 |
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| PRODUCTION OF GABA-ergic NEURONS in vitro OF EMBRYO STEM CELLS AND APPLICATION THEREOF IN TREATING NEURAL DISORDERS | 2004 |
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| GENETIC CONSTRUCT CONTAINING hGDNF CONTROLLED BY TEMPERATURE-SENSITIVE PROMOTOR FOR REGULATED NEUROTROPHIC FACTOR EXPRESSION IN MAMMALIAN CELLS AND BODIES DIRECTLY | 2012 |
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RU2527169C2 |
