METHOD FOR MAKING LOW-MOLECULAR CHITOSAN, SOLUBLE IN ALKALINE ENVIRONMENT Russian patent published in 2010 - IPC C08B37/08 

FIELD: medicine.

SUBSTANCE: invention can be used in making biologically active substances with antibacterial, antimycotic and immunomodulatory properties, and also in designing polymer matrixes for immobilisation of DNA and medical products based on low-molecular chitosan, soluble in alkaline environment. Method for making low-molecular chitosan soluble in alkaline environment characterised by that the implementation thereof involves application of low-molecular chitosan prepared from enzyme depolymerisation and dissolved in an aqueous acid solution. That is followed by addition to the prepared chitosan solution of an alkali solution to pH 7.0 or 7.4. Laid-down chitosan is separated from the solution by centrifugation, and low-molecular chitosan soluble in alkaline environment and contained in a supernatant fluid is lyophilised.

EFFECT: development of the simplified method for making low-molecular chitosan soluble at pH 7,0 and higher, high-yield due to reuse of chitosan fraction insoluble in alkaline environment.

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