METHOD OF PRODUCING LOW-MOLECULAR CHITOSAN IN SALT-FREE MEDIUM VIA ENZYMATIC POLYMERISATION Russian patent published in 2011 - IPC C08B37/08 C12P19/04 

FIELD: chemistry.

SUBSTANCE: starting high-molecular chitosan is dissolved in acid solution. The chitosan dissolved in the acid is then precipitated by adding alkali solution. The re-precipitated high-molecular chitosan is washed from the formed salt and excess alkali using a coarse-porous filter. The re-precipitated chitosan is dissolved in acid solution until achieving pH 5.5. An enzyme preparation is then added and hydrolysis is carried out. The reaction is stopped after formation of low-molecular chitosan.

EFFECT: method is characterised by avoiding the need to remove salts from the enzymatic mixture and the end product, as well as low level of loss of material.

2 dwg, 7 tbl, 6 ex