FIELD: chemistry.
SUBSTANCE: invention relates to analytical chemistry. A method for determining use of corticotropins during doping test in athletes by analysing body fluid samples and detecting glucocorticosteroids is proposed. The method involves taking a urine sample for high-performance liquid chromatography coupled with tandem mass spectrometry and determining content of indirect biochemical markers of intake of corticotropins - cortisol, cortisone and 6βOH-cortisol. If the value of the cortisol/cortisone ratio in the sample is higher than 0.3-1.00 and the 6βOH-cortisol/cortisol ratio is below 15.0, corticotropins have been used.
EFFECT: method cuts the time for testing a group of athletes for doping and expenses for carrying out analysis.
4 ex, 3 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF DETERMINING USE OF ERYTHROPOETIN DURING DOPING TESTS IN ATHLETES | 2008 |
|
RU2390779C2 |
| METHOD OF DETECTING XENOBIOTICS IN HUMAN URINE DURING DOPING TESTS | 2008 |
|
RU2390773C2 |
| METHOD OF DETECTING COMPLEX OF XENOBIOTICS IN BIOLOGICAL FLUID DURING DOPING TEST AND APPARATUS FOR REALISING SAID METHOD | 2011 |
|
RU2473079C1 |
| METHOD TO DETECT DOPING IN HORSES | 2011 |
|
RU2489719C2 |
| METHOD FOR DETECTING HUMAN ENDOGENOUS BLOOD PLASMA STEROIDS | 2010 |
|
RU2451292C2 |
| METHOD FOR IDENTIFICATION AND CLASSIFICATION OF 3-OXOSTEROIDS AND THEIR METABOLITES IN DOPING TEST OF SPORTSMEN | 2010 |
|
RU2452967C2 |
| METHOD FOR MELDONIUM DETERMINATION IN HUMAN URINE | 2017 |
|
RU2639475C1 |
| METHOD FOR RETROSPECTIVE XENOBIOTIC DETECTION ACCOMPANYING SPORTSMEN DOPING CONTROL PROCEDURE | 2011 |
|
RU2478207C1 |
| METHOD FOR DETECTING EXOGENICOUS STEROIDS IN HUMAN BIOLOGICAL FLUID | 2012 |
|
RU2483309C1 |
| METHOD OF DETECTING CONJUGATED XENOBIOTICS DURING DOPING TESTS IN ATHLETES | 2011 |
|
RU2451936C1 |
