FIELD: chemistry.
SUBSTANCE: risk group is made first. Urine samples of athletes undergo high performance liquid chromatography coupled with tandem mass spectrometry. Content of indirect biochemical markers - L-arginine, asymmetrical dimethylarginine, symmetrical dimethylarginine and citrulline is determined. If content of the defined products in the sample is not within 0.9-1.2 of the averaged standard, the athlete is removed from the risk group. If content of the said products is at least greater than 1.5 times the averaged standard, the athlete is included in the risk group. The urine sample of the said athlete is subjected to isoelectric fixation, double blotting and chemoluminescent analysis for exogenous erythropoietin.
EFFECT: method cuts the time for testing a group of athletes for doping and expenses for carrying out analysis.
3 tbl, 4 ex
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Authors
Dates
2010-05-27—Published
2008-07-10—Filed