FIELD: medicine.
SUBSTANCE: there are offered synthetic oligonucleotide primers complementary to highly conserved region of NS1 bluetongue virus genome of the following base composition: 5' -gttaaaaatctatagagatg-3' 5'-gtaagtgtaatctaagaga-3' 5'-acaactgatgctgcgaatga-3' 5'-aacccacacccgtgctaagtgg-3'. There is also disclosed method for detecting bluetongue virus with using such primers and a test system for implementation thereof. The method involves amplification by polymerase chain reaction (PCR). PCR is ensured by using highly conserved region of NS1 bluetongue virus genome. The reaction is considered to be positive if PCR product is matched with the size of a fragment in 800 pairs of bases.
EFFECT: method and test-system can be used in veterinary virology for detecting bluetongue virus.
3 cl, 2 tbl, 5 ex
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| TEST-SYSTEM FOR IDENTIFICATION OF RNA VIRUS OF BLUETONGUE BY RT-PCR METHOD IN REAL TIME MODE | 2012 |
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| SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR IDENTIFICATION OF BLUETONGUE VIRUS OF NUCLEOTYPE C (SEROTYPES 6, 14 AND 21) BY RT-PCR METHOD | 2013 |
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| SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR IDENTIFICATION OF BLUETONGUE VIRUS OF NUCLEOTYPE A (SEROTYPES 4, 10, 11, 17, 20 AND 24) BY METHOD OF RT-PCR | 2013 |
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| SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR IDENTIFICATION OF BLUETONGUE VIRUS OF NUCLEOTYPE B (SEROTYPES 3, 13 AND 16) BY RT-PCR METHOD | 2013 |
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| TEST SYSTEM FOR DETECTING RNA OF SCHMALLENBERG VIRUS AND METHOD FOR RECOGNISING SCHMALLENBERG VIRUS GENOME | 2012 |
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RU2515916C2 |
