FIELD: biotechnologies.
SUBSTANCE: synthetic oligonucleotide primers and probes are disclosed to detect genomes of 1st, 4th and 16th serotypes of the bluetongue disease virus. Also the method is described to detect genomes of 1st, 4th and 16th serotypes of the bluetongue disease virus using such synthetic oligonucleotide primers and probes.
EFFECT: method makes it possible to perform determination of a serotype of the bluetongue disease virus with the help of PCR with a stage of reverse transcription in real time mode, and also to increase specificity and sensitivity, to reduce time to perform work aimed at determination of a serotype of the bluetongue disease virus in samples of an investigated biological material.
4 cl, 4 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| TEST SYSTEM FOR DETECTION OF BLUETONGUE RNA VIRUS OF 14-TH SEROTYPE USING OT-PCR METHOD IN REAL-TIME MODE | 2012 |
|
RU2499054C1 |
| TEST-SYSTEM FOR IDENTIFICATION OF RNA VIRUS OF BLUETONGUE BY RT-PCR METHOD IN REAL TIME MODE | 2012 |
|
RU2510851C1 |
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR IDENTIFICATION OF BLUETONGUE VIRUS OF NUCLEOTYPE C (SEROTYPES 6, 14 AND 21) BY RT-PCR METHOD | 2013 |
|
RU2529955C1 |
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR IDENTIFICATION OF BLUETONGUE VIRUS OF NUCLEOTYPE A (SEROTYPES 4, 10, 11, 17, 20 AND 24) BY METHOD OF RT-PCR | 2013 |
|
RU2534358C1 |
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR IDENTIFICATION OF BLUETONGUE VIRUS OF NUCLEOTYPE B (SEROTYPES 3, 13 AND 16) BY RT-PCR METHOD | 2013 |
|
RU2528745C1 |
| TEST SYSTEM FOR DETECTING RNA OF SCHMALLENBERG VIRUS AND METHOD FOR RECOGNISING SCHMALLENBERG VIRUS GENOME | 2012 |
|
RU2515916C2 |
| OLIGONUCLEOTIDE PRIMERS, DNA-PROBE AND METHOD FOR DETECTION OF VIRUS OF INFECTIOUS ANAEMIA IN HORSES BY METHOD OF PCR OR RT-PCR IN REAL TIME MODE | 2013 |
|
RU2548799C1 |
| TEST SYSTEM FOR DETECTION OF SHEEPPOX VIRUS GENOME BY REAL-TIME POLYMERASE CHAIN REACTION | 2020 |
|
RU2744092C1 |
| OLIGONUCLEOTIDE PRIMERS, METHOD AND TEST SYSTEM FOR DETECTING BLUETONGUE VIRUS GENOME BY POLYMERASE CHAIN REACTION IN ELECTROPHORETIC DETECTION OF AMPLIFICATION PRODUCTS | 2007 |
|
RU2385943C2 |
| OLIGONUCLEOTIDE PRIMERS, FLUORESCENT PROBE AND METHOD FOR RECOGNISING RNA OF IBARAKI DISEASE VIRUS BY REAL-TIME POLYMERASE CHAIN REACTION | 2013 |
|
RU2540142C2 |
