FIELD: chemistry.
SUBSTANCE: invention relates to field of biotechnology. Claimed test-system for identification of RNA virus of bluetongue includes serogroupspecific primers and DNA-probe, complementary sites of conservative 10-th segment, which have the following nucleotide composition (5' - 3'): BTV/10/qf - ACKggTgCWACgCAAACACA; BTV/10/z - FAM - AARgCTgCATTCgCATCgTACGC - BHQ1; BTV/10/qr - ACRTCATCACgAAACgCTTC.
EFFECT: test-system possesses high sensitivity, specificity and quickness in carrying out analysis and makes it possible to identify RNA of bluetongue virus of serotypes by means of RT-PCR method in real time mode.
2 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| TEST SYSTEM FOR DETECTION OF BLUETONGUE RNA VIRUS OF 14-TH SEROTYPE USING OT-PCR METHOD IN REAL-TIME MODE | 2012 |
|
RU2499054C1 |
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR IDENTIFICATION OF BLUETONGUE VIRUS OF NUCLEOTYPE A (SEROTYPES 4, 10, 11, 17, 20 AND 24) BY METHOD OF RT-PCR | 2013 |
|
RU2534358C1 |
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR IDENTIFICATION OF BLUETONGUE VIRUS OF NUCLEOTYPE C (SEROTYPES 6, 14 AND 21) BY RT-PCR METHOD | 2013 |
|
RU2529955C1 |
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR IDENTIFICATION OF BLUETONGUE VIRUS OF NUCLEOTYPE B (SEROTYPES 3, 13 AND 16) BY RT-PCR METHOD | 2013 |
|
RU2528745C1 |
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS-PROBE AND METHOD TO DETECT GENOMES OF 1ST, 4TH, 16TH SEROTYPES OF BLUETONGUE DISEASE VIRUS BY METHOD OF RT-PCR IN REAL-TIME MODE | 2011 |
|
RU2481404C1 |
| OLIGONUCLEOTIDE PRIMERS, FLUORESCENT PROBE AND METHOD FOR RECOGNISING RNA OF IBARAKI DISEASE VIRUS BY REAL-TIME POLYMERASE CHAIN REACTION | 2013 |
|
RU2540142C2 |
| SET OF OLIGODEOXYRIBONUCLEOTIDE PRIMERS AND FLUORESCENT-LABELLED PROBE FOR JMTV RNA IDENTIFICATION BY PCR WITH HYBRIDISATION-FLUORESCENCE DETECTION | 2023 |
|
RU2818960C1 |
| REASSORTANT BTV AND AHSV VACCINES | 2013 |
|
RU2656187C2 |
| OLIGONUCLEOTIDE PRIMERS, METHOD AND TEST SYSTEM FOR DETECTING BLUETONGUE VIRUS GENOME BY POLYMERASE CHAIN REACTION IN ELECTROPHORETIC DETECTION OF AMPLIFICATION PRODUCTS | 2007 |
|
RU2385943C2 |
| OLIGONUCLEOTIDE PRIMERS, METHOD AND TEST SYSTEM FOR DETECTING GENOME OF AFRICAN HORSE SICKNESS VIRUS BY POLYMERASE CHAIN REACTION IN FORMAT OF ELECTROPHORETIC DETECTION OF AMPLIFICATION PRODUCTS | 2007 |
|
RU2378384C2 |
