FIELD: biotechnology.
SUBSTANCE: characterized primers are complementary to sites of variable segment 2 of genome of bluetongue virus of nucleotype C (serotypes 6, 14 and 21) are used in RT-PCR with electrophoretic detection of amplification products for identification of bluetongue virus of serotypes 6, 14 and 21 and have the following composition (5'-3'): direct: GRAYATGRTGGATATWCCG, reverse: GGCTGCACRTCCAYYGARTC.
EFFECT: invention enables to determine the genetic group of nucleotype C of bluetongue virus in samples of the biological material under study using RT-PCR in a short time.
2 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR IDENTIFICATION OF BLUETONGUE VIRUS OF NUCLEOTYPE A (SEROTYPES 4, 10, 11, 17, 20 AND 24) BY METHOD OF RT-PCR | 2013 |
|
RU2534358C1 |
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS FOR IDENTIFICATION OF BLUETONGUE VIRUS OF NUCLEOTYPE B (SEROTYPES 3, 13 AND 16) BY RT-PCR METHOD | 2013 |
|
RU2528745C1 |
| TEST-SYSTEM FOR IDENTIFICATION OF RNA VIRUS OF BLUETONGUE BY RT-PCR METHOD IN REAL TIME MODE | 2012 |
|
RU2510851C1 |
| TEST SYSTEM FOR DETECTION OF BLUETONGUE RNA VIRUS OF 14-TH SEROTYPE USING OT-PCR METHOD IN REAL-TIME MODE | 2012 |
|
RU2499054C1 |
| SYNTHETIC OLIGONUCLEOTIDE PRIMERS-PROBE AND METHOD TO DETECT GENOMES OF 1ST, 4TH, 16TH SEROTYPES OF BLUETONGUE DISEASE VIRUS BY METHOD OF RT-PCR IN REAL-TIME MODE | 2011 |
|
RU2481404C1 |
| OLIGONUCLEOTIDE PRIMERS, METHOD AND TEST SYSTEM FOR DETECTING BLUETONGUE VIRUS GENOME BY POLYMERASE CHAIN REACTION IN ELECTROPHORETIC DETECTION OF AMPLIFICATION PRODUCTS | 2007 |
|
RU2385943C2 |
| OLIGONUCLEOTIDE PRIMERS, FLUORESCENT PROBE AND METHOD FOR RECOGNISING RNA OF IBARAKI DISEASE VIRUS BY REAL-TIME POLYMERASE CHAIN REACTION | 2013 |
|
RU2540142C2 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND FLUORESCENCE-TAGGED PROBE FOR IDENTIFICATION OF RNA OF RIFT VALLEY FEVER VIRUS BY PCR REAL TIME PROCESS | 2014 |
|
RU2552795C1 |
| OLIGONUCLEOTIDE PRIMERS, METHOD AND TEST SYSTEM FOR DETECTING GENOME OF AFRICAN HORSE SICKNESS VIRUS BY POLYMERASE CHAIN REACTION IN FORMAT OF ELECTROPHORETIC DETECTION OF AMPLIFICATION PRODUCTS | 2007 |
|
RU2378384C2 |
| METHOD OF DETECTION AND DIFFERENTIATION OF ENTEROVIRUS RNA | 2000 |
|
RU2189396C2 |
