FIELD: biotechnology, genetic engineering, molecular biology, microbiological and medicinal industry. SUBSTANCE: recombinant plasmid DNA pHIF has fragment Eco RI - Xho I of bacterial-yeast vector pPIC9 and fragment Xho I - Eco RI including encoding moiety of human gene beta-interferon with exception the region encoding signal peptide. Gene encoding beta-interferon is prepared by polymerase chain reaction method and using pHBI template with help of direct and inverse primer. Plasmid pHIF is transformed into the yeast strain to obtain the transformed strain Pichia pastoris PS99 (pHIF). Invention ensures to provide preparing human beta-interferon accumulating in cultural fluid. Invention can be used for preparing human fibroblast interferon. EFFECT: improved method of interferon producing. 4 cl, 2 dwg
| Title |
Year |
Author |
Number |
| STRAIN OF YEAST PICHIA PASTORIS AS PRODUCER OF LEUKOCYTE HUMAN INTERFERON-16, RECOMBINANT PLASMID DNA PHIN AND METHOD FOR ITS CONSTRUCTION |
2002 |
- Padkina M.V.
- Parfenova L.V.
- Sambuk E.V.
- Smirnov M.N.
|
RU2203950C1 |
| STRAIN OF YEAST PICHIA PASTORIS PS105(PBIG) AS PRODUCER OF BOVINE INTRACELLULAR IMMUNE INTERFERON, RECOMBINANT PLASMID DNA PBIG PROVIDING BIOSYNTHESIS OF BOVINE INTRACELLULAR IMMUNE INTERFERON AND METHOD FOR CONSTRUCTING RECOMBINANT PLASMID DNA PBIG |
2002 |
- Gradoboeva A.E.
- Padkina M.V.
- Parfenova L.V.
- Sambuk E.V.
- Smirnov M.N.
|
RU2231545C1 |
| RECOMBINANT PLASMID DNA PHBS PROVIDING BIOSYNTHESIS OF SURFACE ANTIGEN OF HEPATITIS B VIRUS, METHOD OF CONSTRUCTION OF PLASMID, YEAST STRAIN PICHIA PASTORIS PS103 (PHBS) AS PRODUCER OF INDICATED ANTIGEN |
2000 |
- Padkina M.V.
- Parfenova L.V.
- Sambuk E.V.
- Smirnov M.N.
|
RU2201452C2 |
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2006 |
- Gradoboeva Anastasija Evgen'Evna
- Padkina Marina Vladimirovna
- Sambuk Elena Viktorovna
- Smirnov Mikhail Nikolaevich
|
RU2315806C1 |
| STRAIN YEAST PICHIA PASTORIS PS107(pPIC9HAbIL-2) AS PRODUCER OF HYBRID PROTEIN CONSISTING OF HUMAN PLASMA BLOOD ALBUMIN AND HUMAN INTERLEUKIN-2, RECOMBINANT PLASMID pPIC9HAbIL-2 AND METHOD FOR ITS CONSTRUCTING |
2006 |
- Padkina Marina Vladimirovna
- Zinov'Eva Julija Grigor'Evna
- Sambuk Elena Viktorovna
- Smirnov Mikhail Nikolaevich
|
RU2315105C1 |
| PICHIA PASTORIS YEAST STRAIN - PRODUCER OF CHICKEN INTERFERON-GAMMA, RECOMBINANT PLASMID DNA WHICH FACILITATES BIOSYNTHESIS CHICKEN INTERFERON-GAMMA, AND CONSTRUCTION METHOD THEREOF |
2006 |
- Padkina Marina Vladimirovna
- Gradoboeva Anastasija Evgen'Evna
- Sambuk Elena Viktorovna
- Smirnov Mikhail Nikolaevich
|
RU2386694C2 |
| RECOMBINANT PLASMIDE DNA pCPBH FOR BIOSYNTHESIS OF POLYPEPTIDE WITH HUMAN CARBOXYPEPTIDASE B PROPERTIES AND RECOMBINANT STRAIN OF METHYLOTROPHIC YEASTS PICHIA PASTORIS - PRODUCENT OF POLYPEPTIDE WITH HUMAN CARBOXYPEPTIDASE B PROPERTIES |
2011 |
|
RU2531524C2 |
| RECOMBINANT PLASMID DNA DETERMINING SYNTHESIS OF HUMAN FIBROBLAST INTERFERON IN YEAST CELLS, METHOD OF ITS CONSTRUCTION AND YEAST STRAIN SACCHAROMYCES CEREVISIAE AS PRODUCER OF HUMAN FIBROBLAST INTERFERON |
1998 |
- Padkina M.V.
- Parfenova L.V.
- Sambuk E.V.
- Smirnov M.N.
|
RU2180003C2 |
| RECOMBINANT PLASMID DNA pPDGFB ENCODING A POLYPEPTIDE WITH HUMAN PLATELET-BB GROWTH FACTOR HUMAN PROPERTIES, AND A RECOMBINANT STRAIN OF METHYLOTROPHIC YEAST PICHIA PASTORIS, A PRODUCER OF A POLYPEPTIDE WITH HUMAN PLATELET-BB GROWTH FACTOR HUMAN PROPERTIES |
2017 |
- Gerasimov Andrej Sergeevich
- Litvinets Sergej Gennadevich
|
RU2668828C1 |
| RECOMBINANT PLASMID FOR PHYTASE GENE EXPRESSION IN PICHIA PASTORIS YEAST (VERSIONS), PICHIA PASTORIS YEAST STRAIN -PHYTASE PRODUCER (VERSIONS) |
2009 |
- Borshchevskaja Larisa Nikolaevna
- Gordeeva Tat'Jana Leonidovna
- Bavykina Natalija Borisovna
- Sineokij Sergej Pavlovich
|
RU2409670C1 |
