FIELD: biotechnology, molecular biology, genetic engineering.
SUBSTANCE: method involves transformation of the strain yeast Pichia pastoris PS99 (his4 pep4::PHO85) with the constructed plasmid pPIC9HabIL-2 and preparing the strain Pichia pastoris PS107(pPIC9HabIL-2) that represents a producer of hybrid protein consisting of human plasma blood albumin and human IL-2. Invention provides preparing highly stable hybrid protein consisting of human plasma blood albumin and human IL-2 by simplified technology Invention can be used in preparing hybrid proteins preparations.
EFFECT: improved preparing method, valuable properties of strain and hybrid protein.
4 cl, 2 dwg, 5 ex
| Title |
Year |
Author |
Number |
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2000 |
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|
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|
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2006 |
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|
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|
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|
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|
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|
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