FIELD: chemistry; biochemistry.
SUBSTANCE: invention relates to biotechnology, specifically to a method of differentiating Mycoplasma hominis bacteria strains according to the ribosomal RNA gene. Bacteria strains are differentiated according to the ribosomal RNA gene by isolating DNA. Part of the gene is amplified via a polymerase chain reaction with simultaneous introduction of primers MY-RES-F AGTAACACGTGCTTAATCTACCTTT and MY-RES-R CATCGCTTTCTGACAAGGTAC. Further, the obtained amplified part is treated with restriction endonuclease Fsp4HI with subsequent electrophoresis of the obtained DNA fragments in agarose gel. Differentiation is then done by comparing the size of restriction products with control DNA samples.
EFFECT: invention enables detection of epidemic strains of Mycoplasma hominis with different degree of virulence.
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