METHOD OF DETERMINATION OF RADIATION SENSITIVITY OF TUMOUR OF MOUTH MUCOSA Russian patent published in 2010 - IPC A61N5/67 G01N33/52 

FIELD: medicine.

SUBSTANCE: tumour region and surrounding intact tissue receives the diagnostic exposure prior to the series low-intensity lasing source exposure that is carried out at average radiation power 1-10 mWt, wavelength 370 nm with recording the tissue-escaping intensity of back-scattered fluorescence at wavelengths 420, 460 and 520 nm. Thereafter, the tumour region and the surrounding intact tissue are exposed to the low-intensity lasing fluorescence at wavelength 532 nm with recording the tissue-escaping intensity of back-scattered fluorescence at wavelengths 560 and 608 nm. Then the immunohistochemical analysis additionally precedes the treatment with recording the Ki-67 antigen expression level of that tumour region exposed to the low-intensity lasing to determine a prediction criterion P₁ by the initial Ki-67 antigen expression level; there is evaluated the relation of fluorescence intensities at wavelengths 420/460 nm, 460/520 nm, 560/608 nm derived from the tumour surface I_i:K₂=I₄₂₀/I₄₆₀, K₃=I₄₆₀/I₅₂₀, K₄=I₅₆₀/I₆₀₈ and in the intact region I'_i:K'₂=I'₄₂₀/I'₅₂₀, K'₃=I'₄₆₀/I'₅₂₀, K'₄=I'₅₆₀/I'₆₀₈ to calculate the relation of the tumour/intact tissue fluorescence intensity at wavelengths 420/460 nm, 460/520 nm, 560/608 nm: K₂/K'₂, K₃/K'₃, K₄/K'₄; if the Ki-67 antigen expression > 65%, the prediction criteria P₁ are considered to be equal to +1, the expression 45-65% shows P₁=0, the expression <45% requires P₁=-1; the prediction criteria P₂, P₃, P₄ are derived from the results: P₂, P₃, P₄=+1 respectively at K₂/K'₂<1, K₃/K'₃<1, K₄/K'₄<0.5, P₂, P₃, P₄=0 respectively at K₂/K'₂=1-4, K₃/K'₃=1-1.4 K₄/K'₄=0.5-1.5, P₂, P₃, P₄=-1 respectively at K₂/K'₂>4, K₃/K'₃>1.4, K₄/K'₄>1.5, while the radiation sensitivity is determined by total prediction criteria, and P₁+P₂+P₃+P₄>0 indicate high radiation sensitivity, P₁+P₂+P₃+P₄=0 ensures moderate radiation sensitivity, while in P₁+P₂+P₃+P₄<0, low radiation sensitivity is observed.

EFFECT: method simplifies prediction of the results prior to the therapeutic course by tumour sensitivity to ionising radiation, improves its reliability that allows correcting the management program.

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