FIELD: biotechnologies.
SUBSTANCE: food carboxymethylcellulose is dissolved in buffer solution or alkalised distilled water with pH of 9.5-10.0, and cellulose produced by culture Trichoderma reesei or culture Trichoderma viride is added to filtrate of produced solution with activity of at least 50 units/g in the form of powder in the presence of 1-1.5% solution of surfactant in process of intense mixing with speed of 4500-5000 rpm. Produced emulsion is cooled down to 5-10°C, pH of reaction mixture is reduced down to 3.0-3.5 and is left for a day in refrigerator. Precipitated particles are separated from solution, and acetate buffer is added repeatedly to remained particles with pH of 4-4.5 in process of mixing, then precipitated particles that contain cellulase are separated and dried.
EFFECT: reduced dimensions of immobilised cellulase particles, increased speed of fermentative process.
3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR PREPARING IMMOBILISED LIPASE | 2008 |
|
RU2389792C2 |
| METHOD FOR PREPARING IMMOBILISED COLLAGENASE | 2008 |
|
RU2389794C2 |
| METHOD FOR PREPARING IMMOBILISED PROTEASE | 2008 |
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| METHOD OF PRODUCING A HETEROGENEOUS BIOCATALYST BASED ON LIPASE IMMOBILIZED ON KU-2-8 CATION EXCHANGE RESINS IN THE H-FORM | 2023 |
|
RU2813512C1 |
| METHOD OF PRODUCING HETEROGENEOUS BIOCATALYST BASED ON LIPASE IMMOBILIZED ON CATION-EXCHANGE FIBRES “ВИОН КН-1” IN H-FORM | 2023 |
|
RU2818272C1 |
| METHOD OF PRODUCING HETEROGENEOUS BIOCATALYST BASED ON LIPASE IMMOBILIZED ON ANION-EXCHANGE RESINS AV-16-GS AND AN-12P IN OH-FORM | 2023 |
|
RU2823329C1 |
| STRAIN OF MYCELIAL MUSHROOM PENICILLIUM VERRUCULOSUM - PRODUCER OF COMPLEX OF CELLULASE, XYLANASE AND XYLOGLUCANASE AND WAY OF RECEPTION OF FERMENTAL PREPARATION OF COMPLEX OF CELLULASE, XYLANASE AND XYLOGLUCANASE FOR HYDROLYSIS OF CELLULOSE AND HEMICELLULOSE | 2008 |
|
RU2361918C1 |
| METHOD FOR TECHNOLOGICAL PREPARING FERMENTED SUGARS FROM LIGNOCELLULOSE MATERIAL | 2006 |
|
RU2316584C1 |
| FUNGAL STRAIN Penicillium verruculosum B10 EGII PRODUCER OF ENDO-1,3/1,4-β-GLUCANASE, CELLULASE, β-GLUCOSIDASE AND XYLANASE AND METHOD OF PRODUCTION OF FODDER COMPLEX ENZYME PREPARATION | 2012 |
|
RU2532840C2 |
| METHOD FOR ISOLATING CELLULASE | 0 |
|
SU975796A1 |
