FIELD: medicine.
SUBSTANCE: carrier - food grade carboxymethyl cellulose is dissolved in a buffer solution or alkalified distilled water, and filtered. A powdered or concentrated neutral or alkaline protease is introduced in the filtrate with a surfactant solution added while vortexing during 60 minutes. The prepared emulsion is cooled to 5-10°C, then pH of the reaction mixture is reduced to 3.0-3.5, kept for one day in a refrigerator with separating precipitated particles from the solution. An acetate buffer of pH 4-4.5 is added to the stirred remained particles, and then the precipitated particles are separated and dried up.
EFFECT: downsizing the particles of immobilised protease, faster enzymatic process.
3 ex
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